PyroDist

removal of noise from 454 sequenced PCR amplicons

Install

All systems
curl cmd.cat/PyroDist.sh
Debian Debian
apt-get install ampliconnoise
Ubuntu
apt-get install ampliconnoise
image/svg+xml Kali Linux
apt-get install ampliconnoise
Windows (WSL2)
sudo apt-get update sudo apt-get install ampliconnoise
Raspbian
apt-get install ampliconnoise

ampliconnoise

removal of noise from 454 sequenced PCR amplicons

AmpliconNoise is a package of applications to clean up high-throughput sequence data. It consists of three main parts: Pyronoise - does flowgram-based clustering to spot misreads SeqNoise - removes PCR point mutations Perseus - removes PCR chimeras without the need for a set of reference sequences Previously there was a standalone "Pyronoise" by the same authors and this package includes an updated version. There is also a "Denoiser" in Qiime which is related but distinct.