crb-blast
Run conditional reciprocal best blast
Install
- All systems
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curl cmd.cat/crb-blast.sh
- Debian
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apt-get install ruby-crb-blast - Ubuntu
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apt-get install ruby-crb-blast - Kali Linux
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apt-get install ruby-crb-blast - Windows (WSL2)
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sudo apt-get updatesudo apt-get install ruby-crb-blast - Raspbian
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apt-get install ruby-crb-blast - Dockerfile
- dockerfile.run/crb-blast
ruby-crb-blast
Run conditional reciprocal best blast
CRB-BLAST is a novel method for finding orthologs between one set of sequences and another. This is particularly useful in genome and transcriptome annotation. CRB-BLAST initially performs a standard reciprocal best BLAST. It does this by performing BLAST alignments of query->target and target->query. Reciprocal best BLAST hits are those where the best match for any given query sequence in the query->target alignment is also the best hit of the match in the reverse (target->query) alignment. Reciprocal best BLAST is a very conservative way to assign orthologs. The main innovation in CRB-BLAST is to learn an appropriate e-value cutoff to apply to each pairwise alignment by taking into account the overall relatedness of the two datasets being compared. This is done by fitting a function to the distribution of alignment e-values over sequence lengths. The function provides the e-value cutoff for a sequence of given length.