long_seq_tm_test
tool to design flanking oligo nucleotides for DNA amplification
Install
- All systems
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curl cmd.cat/long_seq_tm_test.sh
- Debian
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apt-get install primer3 - Ubuntu
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apt-get install primer3 - Kali Linux
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apt-get install primer3 - Fedora
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dnf install primer3 - Windows (WSL2)
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sudo apt-get updatesudo apt-get install primer3 - OS X
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brew install primer3 - Raspbian
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apt-get install primer3 - Dockerfile
- dockerfile.run/long_seq_tm_test
primer3
tool to design flanking oligo nucleotides for DNA amplification
Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.