ntdpal

tool to design flanking oligo nucleotides for DNA amplification

Install

All systems
curl cmd.cat/ntdpal.sh
Debian Debian
apt-get install primer3
Ubuntu
apt-get install primer3
image/svg+xml Kali Linux
apt-get install primer3
Fedora
dnf install primer3
OS X
brew install primer3
Raspbian
apt-get install primer3

primer3

tool to design flanking oligo nucleotides for DNA amplification

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.