ntdpal

tool to design flanking oligo nucleotides for DNA amplification

Install

All systems
curl cmd.cat/ntdpal.sh
Debian Debian
apt-get install primer3
Ubuntu
apt-get install primer3
image/svg+xml Kali Linux
apt-get install primer3
Fedora
dnf install primer3
Windows (WSL2)
sudo apt-get update sudo apt-get install primer3
OS X
brew install primer3
Raspbian
apt-get install primer3
Dockerfile
dockerfile.run/ntdpal

primer3

tool to design flanking oligo nucleotides for DNA amplification

Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as criteria oligonucleotide melting temperature, size, GC content and primer-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints. All of these criteria are user-specifiable as constraints, and some are specifiable as terms in an objective function that characterizes an optimal primer pair.