perlprimer

Graphical design of primers for PCR

Install

All systems
curl cmd.cat/perlprimer.sh
Debian Debian
apt-get install perlprimer
Ubuntu
apt-get install perlprimer
image/svg+xml Kali Linux
apt-get install perlprimer
Windows (WSL2)
sudo apt-get update sudo apt-get install perlprimer
Raspbian
apt-get install perlprimer

perlprimer

Graphical design of primers for PCR

PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard Polymerase Chain Reaction (PCR), bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design. If operated online, the tool nicely communicates with the Ensembl project for further insights into the gene structure, i.e., allowing for taking the location of exons and introns into account for the design of the primers. The sequences themselves can be retrieved, too.