seqprep

stripping adaptors and/or merging paired reads of DNA sequences with overlap

Install

All systems
curl cmd.cat/seqprep.sh
Debian Debian
apt-get install seqprep
Ubuntu
apt-get install seqprep
image/svg+xml Kali Linux
apt-get install seqprep
Windows (WSL2)
sudo apt-get update sudo apt-get install seqprep
Raspbian
apt-get install seqprep

seqprep

stripping adaptors and/or merging paired reads of DNA sequences with overlap

SeqPrep is a program to merge paired end Illumina reads that are overlapping into a single longer read. It may also just be used for its adapter trimming feature without doing any paired end overlap. When an adapter sequence is present, that means that the two reads must overlap (in most cases) so they are forcefully merged. When reads do not have adapter sequence they must be treated with care when doing the merging, so a much more specific approach is taken. The default parameters were chosen with specificity in mind, so that they could be ran on libraries where very few reads are expected to overlap. It is always safest though to save the overlapping procedure for libraries where you have some prior knowledge that a significant portion of the reads will have some overlap.