seqprep
stripping adaptors and/or merging paired reads of DNA sequences with overlap
Install
- All systems
-
curl cmd.cat/seqprep.sh
- Debian
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apt-get install seqprep - Ubuntu
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apt-get install seqprep - Kali Linux
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apt-get install seqprep - Windows (WSL2)
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sudo apt-get updatesudo apt-get install seqprep - Raspbian
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apt-get install seqprep - Dockerfile
- dockerfile.run/seqprep
seqprep
stripping adaptors and/or merging paired reads of DNA sequences with overlap
SeqPrep is a program to merge paired end Illumina reads that are overlapping into a single longer read. It may also just be used for its adapter trimming feature without doing any paired end overlap. When an adapter sequence is present, that means that the two reads must overlap (in most cases) so they are forcefully merged. When reads do not have adapter sequence they must be treated with care when doing the merging, so a much more specific approach is taken. The default parameters were chosen with specificity in mind, so that they could be ran on libraries where very few reads are expected to overlap. It is always safest though to save the overlapping procedure for libraries where you have some prior knowledge that a significant portion of the reads will have some overlap.