seqtk

Fast and lightweight tool for processing sequences in the FASTA or FASTQ format

Install

All systems
curl cmd.cat/seqtk.sh
Debian Debian
apt-get install seqtk
Ubuntu
apt-get install seqtk
image/svg+xml Kali Linux
apt-get install seqtk
Windows (WSL2)
sudo apt-get update sudo apt-get install seqtk
OS X
brew install seqtk
Raspbian
apt-get install seqtk

seqtk

Fast and lightweight tool for processing sequences in the FASTA or FASTQ format

Currently, seqtk supports quality based trimming with the phred algorithm, converting fastq to fasta, reverse complementing sequences, extracting or masking subsequences in regions given in a BED/name list file, and more. It contains a subsampling module to sample exactly n sequences or a fraction of sequences. Seqtk supports both fasta and fastq input files, which can be optionally gzip compressed.